The National Museum of Ancient Art (Museu Nacional de Arte Antiga) is a museum in Lisbon, Portugal that houses an extensive collection of Portuguese art from the 12th to the 19th centuries, as well as European and non-European art from the same period.

The museum's collection includes painting, sculpture, textiles, decorative arts, gold and silverware, and furniture, among other art forms. The Portuguese collection is particularly noteworthy, with works by some of the country's most important artists, such as Nuno Gonçalves, Vasco Fernandes, and Josefa de Óbidos. There are also notable pieces from international artists such as Hieronymus Bosch, Albrecht Dürer, and Hans Holbein the Younger.

Some of the museum's highlights include the "St. Vincent Panels" by Nuno Gonçalves, which are considered a masterpiece of Portuguese painting, and the "Triptych of the Temptation of St. Anthony" by Hieronymus Bosch, which is one of the artist's most famous works.

The museum is housed in a 17th-century palace overlooking the Tagus River and includes a garden with a view of the river. In addition to its permanent collection, the museum also hosts temporary exhibitions, educational activities, and cultural events throughout the year.